Further Studies on the Polytene Chromosomes of Stylonychia mytilus (Ciliata, Hypotrichia).

Release of Dna Breakdown Products into the Culture Medium of Stylonychia Mytilus Exconjugants (protozoa, Ciliata) during the Destruction of the Polytene Chromosomes

1000 anlage was labeled by adding 400 Mc/ml thymidineH (Nuclear-Chicago Corp., Des Plaines, Ill. spec. act., 17, 2 mc/mM) to a culture of exconjugants during the first polyploidization stage (0-22 hr after separation of the conjugation pairs). After this, the exconjugants were washed three times with culture fluid, placed for 1 hr in fresh culture fluid, followed by 1 hr in culture fluid with

Distribution studies on polytene chromosomes using antibodies directed against hnRNP

The distribution of nuclear ribonucleoprotein (hnRNP) particles in Drosophila polytene chromosomes has been investigated using anti-B-36 serum as a probe. The use of polytene chromosomes allows resolution at the level of the chromomere, and provides the opportunity to look for both positive and negative correlations with transcriptional activity. The antiserum was obtained using the nuclear pro...

zeste protein on polytene chromosomes

Gravikinesis in Stylonychia mytilus is based on membrane potential changes.

The graviperception of the hypotrichous ciliate Stylonychia mytilus was investigated using electrophysiological methods and behavioural analysis. It is shown that Stylonychia can sense gravity and thereby compensates sedimentation rate by a negative gravikinesis. The graviresponse consists of a velocity-regulating physiological component (negative gravikinesis) and an additional orientational c...

Ultrastructural localization of chitin in the cystic wall of Euplotes muscicola Kahl (ciliata, hypotrichia).

The ultrastructural localization of chitin in the cyst walls of Euplotes muscicola was carried out after deproteinization and by specific labelling with WGA-gold complex. The overall ectocyst contains chitin that alone can sustain the exact profile of this cystic layer. In the endocyst, electron clear sublayers include chitin and alternate with other sublayers probably of proteic nature.